Short answer: I don't have a real joeverse currently, so I can't say for sure.

Longer:

Depends on how treacherous they are and what they'd gain. If Destro was mad at C.C. or not or had his own army doing its own thing.

The only sure thing is that Serpentor has Mindbender in his corner. Cobra Commander's treachery towards his own lackeys means that any of them could side against him and side with a rival more out of hatred towards CC than loyalty to someone else.

Taking the comic into consideration, who doesn't have reasons to want C.C. deposed or dead? (Mostly minor players like Wild Weasel, Slice and Dice)

Taking the cartoons into consideration, who never betrayed the Commander? (Copperhead, Wild Weasel, Firefly, Storm Shadow and Major Bludd) Who restored him, despite earlier betrayals? (Baroness...with Gnawgahyde).

The toys, Mindbender's role in the V-Troops and BAT program would put related troops on characters on Serpentor's side perhaps. The Crimson Guard are supposed to Cobra Commander's most loyal troopers.

Most fans favor Cobra Commander so much that when a split comes around, they usually lump the main Cobra characters all on the Commander's side. Serpentor gets nobodies (Overlord) and not-so-favorites and Dr. Mindbender. This allows a reset button, so once the conflict is over, Cobra's mainstays remain.

But it flies in the face of the Sunbow series and the comic, where betrayal was the hallmark of Cobra's golden years. The Hasbro/Sunbow rationale for Serpentor's creation was all about his creators gaining power. I suspect that was Dr. Venom's original plan in the comic (he came up with the idea of a composite clone super trooper) , since he was quite treacherous.